УПРАВЛЕНИЕ В МЕДИЦИНЕ И БИОЛОГИИ UDC 543.2 doi:10.15217/issn1684-8853.2015.3.107 FEASIBILITY STUDY — VITAMIN D LOADING DETERMINATION BY FTIR-ATR E. Simanaa, B.Sc., Electrical Engineering, eran.simana@gmail.com R. Simiana, B.Sc., Electrical Engineering, ronensimian@gmail.com S. Portnoyb, PhD, Biomedical Engineering, portnoys@tauex.tau.ac.il A. Jaffec, M.D, PhD, Endocrinologist, jaffe@hy.health.gov.il B. Z. Dekela, PhD, Electrical Engineering, benziond@ruppin.ac.il aRuppin Academic Center, Emek-Hefer, 40250, Israel bOccupation Therapy Department, Tel-Aviv University, Tel-Aviv, Israel cEndocrinology and Diabetes Unit, Hillel Yaffe Medical Center, Hadera, Israel 16 days after they took a dose of 200,000 IU vitamin D3. <...> Samples were measured by the conventional bio-chemical method and by the evanescent wave spectroscopy means. <...> Results: Correlation was found between the vitamin D levels measured by the traditional method and by the evanescent wave spectroscopy technique. <...> The absorption lines occurred prominently in the IR spectral regions of the Amide I (≈1650 cm−1),Amide II (≈1530 cm−1) and the (≈3400 cm−1) absorption band which is attributed to the hydroxyl group indicated by the O-H stretch. <...> In addition, the examination of the blood samples using the evanescent wave spectroscopy with clustering techniques facilitated the discrimination between vitamin D deficiency and normal vitamin D levels. <...> Practical relevance: This study demonstrates the potential of using the Fourier transform infra red method in conjugation with the evanescent wave spectroscopy techniquecoupled with multivariate analysis as a nonexpensive, rapid and accurate alternative to the routine methodologies. <...> Keywords — FTIR-ATR Spectroscopy, Mid Infrared, Vitamin D. Introduction It has been well-known for decades that vitamin D is activated in the skin by ultraviolet B radiation and that its main activities are calcium balanceby affectingcalciumabsorption, secretion, bone metabolism and control of PTH. <...> In the last decade, it has been suggested that vitamin D plays an important role in various diseases, e.g. osteoporosis, rickets, diabetes mellitus, cardiovascular disease, multiple sclerosis, different forms of cancer and a number of mental disorders, including depression [2]. <...> Vitamin D exists in two main physiological forms [3], vitamin D2 <...>