Журнал Сибирского федерального университета. Биология. Journal of Siberian Federal University/ Biology №2 2008

Стр.1

Стр.2

Стр.3

Журнал Сибирского федерального университета Journal of Siberian Federal University Биология Biology Редакционный совет академик РАН Е.А.Ваганов академик РАН К.С.Александров академик РАН И.И.Гительзон академик РАН В.Ф.Шабанов чл.-к. РАН, д-р физ.-мат.наук А.Г.Дегерменджи чл.-к. РАН, д-р физ.-мат. наук В.Л.Миронов чл.-к. РАН, д-р техн. наук Г.Л.Пашков чл.-к. РАН, д-р физ.-мат. наук В.В.Шайдуров чл.-к. РАО, д-р физ.-мат. наук В.С. Соколов Editorial Advisory Board Chairman: Eugene A. Vaganov Members: Kirill S. Alexandrov Josef J. Gitelzon Vasily F. Shabanov Andrey G. Degermendzhy Valery L. Mironov Gennady L. Pashkov Vladimir V. Shaidurov Veniamin S. Sokolov Editorial Board: Editor-in-Chief: Mikhail I. Gladyshev Founding Editor: Vladimir I. Kolmakov Managing Editor: Olga F. Alexandrova Executive Editor for Biology: Nadezhda N.Sushchik Компьютерная верстка И.В. Гревцовой Подписано в печать 27.08.2008 г. Формат 19х27. Усл. печ. л. 7,7. Уч.-изд. л. 7,5. Бумага тип. Печать офсетная. Тираж 1000 экз. Заказ 1/062. Отпечатано в ИПК СФУ. 660041 Красноярск, пр. Свободный, 79. Anna S. Tarasova, Nadezhda S. Kudryasheva, Elena S. Fedorova, Devard I. Stom Bioluminescent Monitoring: Detoxification of Phenol Solutions by Humic Substances and UV-irradiation – 136 – CONTENTS / СОДЕРЖАНИЕ Denis Y. Rogozin and Andrey G. Degermendzhi Hydraulically-operated Thin-layer Sampler for Sampling Heterogeneous Water Columns – 111 – Natalia A. Tikhomirova, Sofya A. Ushakova, Alexander A. Tikhomirov, Galina S. Kalacheva and Jean-Bernard Gros Salicornia europaea L. (fam. Chenopodiaceae) Plants as Possible Constituent of Bioregenerative Life Support Systems’ Phototrophic Link – 118 – Tatiana G. Volova, Sergei A. Gordeev and Ekaterina I. Shishatskaya Production of Oriented Fibers out of Poly(Hydroxybutyrate/ Hydroxyvalerate) Copolymers and Testing of Mechanical Stability under Static and Cyclic Loads – 126 – 2008 1 (2)

Стр.1

Editorial board for Biology: Sergey I. Bartsev Alexander Y. Bolsunovsky Tatiana G. Volova Eugene S. Vysotski Nikolai A. Gaevsky Egor S. Zadereev Valentina A. Kratasyuk Elena N. Muratova J. Woodland Hastings Frank D. Salisbury Malcolm K. Hughes Ernst-Detlef Schulze Akira Osawa Takayoshi Koike Marc D’Alarcao Ольга П. Дубовская Оценка количества мертвых особей рачкового зоопланктона в водоеме с помощью окрашивания проб анилиновым голубым: методические аспекты применения – 145 – Сергей И. Сиделев, Ольга В. Бабаназарова Анализ связей пигментных и структурных характеристик фитопланктона высокоэвтрофного озера – 162 – Review Krasnoyarsk Water Reservoir: Monitoring, Biota, Water Quality Свидетельство о регистрации СМИ ПИ № ФС77-28-725 от 29.06.2007 г. – 178 –

Стр.2

Denis Y. Rogozin and Andrey G. Degermendzhi. Hydraulically-operated Thin-layer Sampler for Sampling… Journal of Siberian Federal University. Biology 2 (2008 1) 111-117 ~ ~ ~ УДК 578.763 Hydraulically-operated Thin-layer Sampler for Sampling Heterogeneous Water Columns Denis Y. Rogozina, * and Andrey G. Degermendzhia,b a Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia b Received 2.06.2008, received in revised form 25.08.2008, accepted 27.08.2008 A necessary condition for investigating microbial communities in chemocline zones of stratified waterbodies is high-precision sampling at a depth resolution of several centimeters. We have modified a multi-syringe sampler that is conventionally used by researchers for this purpose. The main distinctive feature of our sampler is that plungers are operated hydraulically rather than pneumatically and, thus, our sampler has the following advantages: (1) the plungers cannot move spontaneously, which ensures sampling adequacy, especially in deep-water sampling; (2) filling of the syringes with the sample can be monitored from a distance; (3) during storage, the internal volume of the syringe and the whole hydraulic system is aseptic (if 70% ethanol is used as a working fluid), which is important if samples are to be used for microbiological tests. (4) the problem of sticking plungers is minimized, even after extended storage. Vertical sampling of the chemocline zones of meromictic lakes in Southern Siberia (Russia, Khakasia) at a depth resolution of 5 cm proves that the sampler is very reliable and simple to use. Keywords: chemocline, microstratification, thin-layer sampler. Introduction In ecosystems of stratified waterbodies, a considerable contribution to material and energy fluxes is made by processes occurring in the zones of sharp physicochemical gradients of the water column, i.e. in the thermocline, the chemocline, and the picnocline. Much research has been done on planktonic communities and processes in the gradient zones of stratified lakes (e.g, Jorgensen et al., 1975; Overmann, 1997; Tonolla et al., 2003; and many others), but the structure and functions of these communities and * Corresponding author E-mail address: rogozin@ibp.ru 1 © Siberian Federal University. All rights reserved – 111 – their ecological role in the waterbodies are still underinvestigated. Moreover, stratified lakes are invariably attractive to researchers as “natural laboratories” in which different ecological niches of plankton microorganisms are formed along such physicochemical gradients as temperature, redox conditions, salinity, density, etc. It is well known that an adequate insight into biochemical processes and biota in the gradient zones of small lakes can only be provided by high-precision sampling at a depth resolution of several centimeters. It was reported that use of conventional samplers led to a Siberian Federal University, 79 Svobodny, Krasnoyarsk, 660041 Russia 1

Стр.3