ПАРАЗИТОЛОГИЯ, 51, 1, 2017 УДК 632.651 THE SIMPLEST «FIELD» METHODS FOR EXTRACTION OF NEMATODES FROM PLANTS, WOOD, INSECTS AND SOIL, WITH ADDITIONAL DESCRIPTION HOW TO KEEP EXTRACTED NEMATODES ALIVE FOR A LONG TIME © A. Yu. <...> Ryss Zoological Institute RAS Universitetskaya naberezhnaya 1, St Petersburg, 199034 E-mail: nema@zin.ru Submitted 01.10.2016 The simplest modification of the dynamic extraction method using cottonwool filter based on the Baermann funnel principle, is described. <...> This modification excludes the funnel because a great share of sticky worms attach to sloping walls of a funnel and thus do not reach the collector Eppendorf tube. <...> In the proposed modification any sloping walls in the extraction paths are excluded and thus the probability to attach sticky nematodes to walls is also excluded; only cylindrical equipment with abrupt vertical walls is used; procedures are extremely simplified to be user-friendly for beginners: only filter (cotton pads), Eppendorf tubes, plastic glasses and narrow PVC tubing are applied. <...> The new simplified modification allows one to collect nematodes by non-professional workers, e. g. in Polar expeditions without microscopic study of results. <...> As an addition, an efficient method to maintain extracted nematodes alive is proposed, using the «effect of water film» in foam rubber inside the Eppendorf tube. <...> To maintain nematodes alive during several months it is recommended to suppress bacteria via addition of 0.2—0.4% formaldehyde solution and then keep the tube with nematodes in a refrigerator. <...> Key words: nematodes, field extraction technique, nematode collection in Polar expedition, Baermann funnel modification, alive nematode storage. 57 САМАЯ ПРОСТАЯ МЕТОДИКА ПОЛЕВОЙ ЭКСТРАКЦИИ НЕМАТОД ИЗ РАСТЕНИЙ, НАСЕКОМЫХ И ПОЧВЫ, А ТАКЖЕ МЕТОДИКА ДЛИТЕЛЬНОГО СОХРАНЕНИЯ ЭКСТРАГИРОВАННЫХ НЕМАТОД ЖИВЫМИ © А. <...> Methods of extraction of nematodes, i. e. separation of roundworms from the substratum, may be divided in three groups: centrifugal, dynamic and flotation ones (Van Bezooijen, 2006; OEPP/EPPO 2013; Ryss, 2015; De Ley et al, 2016). <...> Centrifugal methods are based on the mass density (specific gravity) of the extraction solution, which may be equal <...>