AGRICULTURAL BIOLOGY, 2016, V. 51, ¹ 5, pp. 654-663 Plant and soil UDC 573.22:581.55 doi: 10.15389/agrobiology.2016.5.654rus doi: 10.15389/agrobiology.2016.5.654eng METAGENOMIC CHARACTERISTIC OF RHIZOSPHERE EFFECT ON CEREALS IN BLACK AND SOD-PODZOLIC SOILS A.O. ZVEREV, E.V. PERSHINA, N.A. PROVOROV, E.E. ANDRONOV, E.N. SERIKOVA All-Russian Research Institute for Agricultural Microbiology, Federal Agency of Scientific Organizations, 3, sh. <...> Podbel’skogo, St. Petersburg, 196608 Russia, e-mail azver.bio@gmail.com Acknowledgements: Supported by Russian Science Foundation (project 14-26-00094) Received July 6, 2016 A b s t r a c t Сhanges in the composition of microbial communities under the influence of root exudation of plants (rhizosphere effect) is widely reported in the scientific literature. <...> A number of studies clearly show the rhizosphere effect of external factors such as soil type, species and plant variety, etc. <...> The aim of this work is to study the effect of soil type and plant species using modern highthroughput sequencing techniques. <...> This effect has been studied well by foreign counterparts, but such work on Russian soils and crops used in the domestic agro-industry, is carried out for the first time. <...> We used two soils contrasting by their agrochemical parameters, black earth (Voronezh region), and sod-podzolic soil (Pskov region). <...> Rye (Secale cereale L., k-6469) and wheat (Triticum aestivum L., k54609) seeds obtained from VIR collection (St. Petersburg) were grown in a greenhouse on both soils for 42 days. <...> Using NGS-V4 variable region sequenced 16S rDNA gene, microbial community composition in bulk soils and the rhizospheres formed on them was analyzed. <...> Despite the short period of the experiment, clear rhizosphere effect was revealed in both soils. <...> Communities of bulk soil as well as rhizosphere communities on these soils, were significantly different from each other. <...> Both soils show the same effect in the formation of rhizosphere communities of rye and wheat. <...> Type of plant is the second largest (after the type of soil) factor in determining taxonomic composition of the rhizosphere microbiome. <...> Also, the rhizosphere communities of rye on sod-podzolic soil according to the cluster analysis are closer in structure to the original communities of the soil. <...> The taxonomic analysis of the communities at the level of phyla <...>