Национальный цифровой ресурс Руконт - межотраслевая электронная библиотека (ЭБС) на базе технологии Контекстум (всего произведений: 635254)
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Вестник Астраханского государственного технического университета. Серия: Рыбное хозяйство  / №2 2014

EFFECT OF MAGNETIC STORMS ON THE TEMPERATURE CHARACTERISTICS OF DIGESTIVE GLYCOSIDASES IN ROACH FINGERLINGS (90,00 руб.)

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Первый авторFilippov
АвторыKrylov V.V., Golovanovа I.L.
Страниц5
ID504452
АннотацияThe aftereffects of a typical geomagnetic storm (the duration 24 h, in the frequency range 0–5 Hz) on the activity of glycosidase (maltase, amylolytic activity) in the intestine of a 4-month juvenile roach Rutilus rutilus (L.) have been studied. Embryos were exposed to geomagnetic storm 72 h later after fertilization. The amylolytic activity was determined by Nelson method and the maltase activity by the glucose-oxidase method. The level of glycosidase activity in juvenile roach under the impact of geomagnetic storm was significantly lower than that in controls. Temperature characteristics of glycosidase in fish of the experimental and the control groups are similar. Temperature optimum of amylolytic activity was observed at 40 єC, the temperature optimum of maltase was found at 60 єC. The Eact values of maltase at the temperature 0-30 °C are similar in both groups of yearlings. The Eact values of amylolytic activity at feeding temperature 10-20 °C were significantly lower (the efficiency of the process is higher) than at lower temperatures in both groups of yearlings
УДК597.554.3-154.31
Filippov, A.A. EFFECT OF MAGNETIC STORMS ON THE TEMPERATURE CHARACTERISTICS OF DIGESTIVE GLYCOSIDASES IN ROACH FINGERLINGS / A.A. Filippov, V.V. Krylov, I.L. Golovanovа // Вестник Астраханского государственного технического университета. Серия: Рыбное хозяйство .— 2014 .— №2 .— С. 102-106 .— URL: https://rucont.ru/efd/504452 (дата обращения: 14.05.2024)

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Физиология и биохимия гидробионтов UDC 597.554.3-154.31 A. A. Filippov, V. V. Krylov, I. L. Golovanovа EFFECT OF MAGNETIC STORMS ON THE TEMPERATURE CHARACTERISTICS OF DIGESTIVE GLYCOSIDASES IN ROACH FINGERLINGS1 Abstract. <...> The aftereffects of a typical geomagnetic storm (the duration 24 h, in the frequency range 0–5 Hz) on the activity of glycosidase (maltase, amylolytic activity) in the intestine of a 4-month juvenile roach Rutilus rutilus (L.) have been studied. <...> Temperature characteristics of glycosidase in fish of the experimental and the control groups are similar. <...> Temperature optimum of amylolytic activity was observed at 40 ºC, the temperature optimum of maltase was found at 60 ºC. The Eact values of maltase at the temperature 0−30 °C are similar in both groups of yearlings. <...> The Eact values of amylolytic activity at feeding temperature 10−20 °C were significantly lower (the efficiency of the process is higher) than at lower temperatures in both groups of yearlings. <...> Introduction The geomagnetic storm (GMS) is a disturbance of the geomagnetic field, associated with an interaction of the perturbed flow of the solar wind and the Earth's magnetosphere. <...> The intensity of geomagnetic fluctuations during GMS does not often exceed 1 % of the intensity of the geomagnetic field. <...> Aftereffects of a real GMS reproduced in experimental conditions, on the early stages of roach development manifested as an increase in the mitotic activity in prelarvae cells [2], the decrease in size-weight characteristics and in vertebral phenotypes diversity in juveniles [3]. <...> Multidirectional changes in the activity of glycosidase (enzyme hydrolyzing carbohydrate) were previously found in the gut of juvenile roach subjected to the effect of lowfrequency magnetic field during its embryogenesis [4, 5]. <...> At the same time, there is no information about the aftereffect of the magnetic storm on the digestive function of fish. <...> The goal of the present work is to study the prolonged effects of GMS exposure during embryogenesis upon activities and temperature characteristics of intestinal glycosidase in roach fingerlings. <...> Materials and methods of research The fertilized eggs obtained from the parental roach Rutilus rutilus (L.) (8 females and 6 males) caught in May 2012 at the Rybinsk Reservoir spawning grounds were used for the experiments. <...> The geomagnetic storm (happened on 30–31 October 2003 and recorded at the latitude of the experiments) was reproduced in the experimental setup that allows compensating <...>