Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2011, № 5, p. 45-50 UDC 635.649:577.29:57.2.08 POLYMORPHISM OF MICROSATELLITE LOCUS CAMS-336 IN PEPPER VARIETIES AND CLOSELY RELATED SPECIES 2 All-Russia Research and Development Institute of Vegetable Crops Selection and Seed Growing, RAAS, Moscow province, Odintsovo region, Lesnoy Gorodok 143080, Russia e-mail: pishnaya_o@mail.ru E.A. Snigir’1, 2, O.N. Pyshnaya2, E.Z. Kochieva1, 3, N.N. Ryzhova1 1 Scientific center “Bioengineering”, RAS, Moscow 117312, Russia e-mail: ekochieva@yandex.ru 3 M.V. Lomonosov Moscow State University, Moscow 119899, Russia Received October 19, 2010 S u m m a r y The polymorphism of microsatellite locus CAMS-336 was investigated in 45 pepper varieties of native and foreign selection, and also in variants of closely related cultivated species of the Сapsicum frutescens, C. chinense, C. baccatum. <...> The unique alleles for some varieties and species were isolated and the PIC quantities for complete set of variants were calculated. <...> The allelic variability was confirmed by sequencing, which permitted to determine the accurate nucleotide sequence and the size of revealed alleles of microsatellite locus CAMS-336. <...> In addition, the sequencing of this locus permits to determine the mechanism of appearance of new B and D alleles, notably point substitution of T for A. The obtained data suggests the possibility of the use of microsatellite locus CAMS-336 for issue passports to the native varieties of C. аnnuum L. Keywords: SSR analysis, Сapsicum аnnuum, cultivar fingerprinting. <...> Identification and certification of cultivated plant varieties is one of the main ways to protect intellectual property rights in plant breeding and seed production. <...> Currently, this problem is being widely solved by molecular labeling of genomes, primarily using SSR-analysis (simple sequence repeats) of microsatellite loci - short (less than 6 nucleotides) tandemly repeated DNA sequences. <...> Such repeats are found in both hetero- and euchromatic regions of plant genomes including exons, introns and intergenic sequences; as a rule, they are uniformly distributed over the genome (1). <...> Variable number of repeats within a microsatellite is the result of DNA slippage during replication or unequal crossing-over (4). <...> The analysis of allelic polymorphism <...>