BAIMIEV Ufa Scientific Center of Russian Academy of Sciences, 71, prosp. <...> Oktyabrya, Ufa, Republic of Bashkortostan, 450054 Russia, e-mail blagova_darya@mail.ru Supported by Special Federal Program and Russian Foundation for Basic Research A b s t r a c t Biomethods in plant protection against pests and diseases considered the most prospective alternative to chemicals which pollute soil and water causing concern about public health. <...> The possibility of creating an artificial association of nodule bacteria with plants to protect them from the adverse effects of pathogenic fungi can be realized using one of the specific mechanism of nodule bacteria attachment to the roots of leguminous plants by plant lectins, able to recognize and specifically bind with different carbohydrates, particularly polysaccharides of rhizobia cell wall. <...> In our study we used the composite plants of tomato ( ) Dubok variety and bacterial strains associated with the roots of wild legumes from the collection of ment with 1305.1 with inserted pea lectin gene (IBG USC RAS). «Hairy rooted» tomato plants were obtained by treatATCC 15834, containing vehicle gene construction pCambia under cauliflower mosaic virus 35S promoter. <...> The antagonistic activity of bacteria towards pathogens was tested by dual culture study. <...> The ability of microorganisms to produce siderophores and cyanide was analyzed. <...> Few isolates were identified by sequencing of the 16S rRNA gene fragments. <...> By screening of the collection of isolates from nodules of wild legume from tribe the candidate strains were detected, particularly sp. and sp. and siderophores was detected in two members of . <...> It was also found that treatment of roots transgenic by strain reduced the amount of hyphae of the pathogen f. sp. , with fungistatic activity against . <...> Prodiction of and gene with were shown to produce 116 sp. in the rhizosphere of tomato plant that could potentially contribute to plant defense against pathogenic fungi. <...> Thus, the use of lectins as transgenes in roots allows us to obtain artificial association with rhizobia in non-symbiotic plants such as tomato, which in combination with the use of microorganisms possessing fungistatic activity can more effectively protect the plant root system o against pathogens. <...> Biological methods of plant protection provide an alternative to chemical techniques. <...> The colonization of nonleguminous plant roots <...>