AGRICULTURAL BIOLOGY, 2016, V. 51, ¹ 1, pp. 60-67 ISSN 2412-0324 (English ed. Online) (SEL’SKOKHOZYAISTVENNAYA BIOLOGIYA) ISSN 0131-6397 (Russian ed. Print) ISSN 2313-4836 (Russian ed. Online) SSR-genotyping to control the origin doi: 10.15389/agrobiology.2016.1.60rus doi: 10.15389/agrobiology.2016.1.60eng GENETIC SIMILARITY OF THE AUTOCHTHONOUS GRAPEVINE VARIETIES FROM DON REGION REVEALED BY SSR-ANALYSIS AND MAIN LEAF AMPELOGRAPHIC TRAITS E.T. IL’NITSKAYA1, S.V. TOKMAKOV1, I.I. SUPRUN1, L.G. NAUMOVA2, V.A. GANICH2 , Federal Agency of Scientific Organizations, 39, ul. <...> Many native Don grape varieties represent a significant value for cultivation and use in breeding. <...> The main features of the leaves of grape varieties are the key ampelographical characteristics. <...> Currently, the study at the DNA level is considered the most informative method of plant genotyping analysis. <...> Microsatellite markers are widely used for genotyping of grapevine varieties and rootstocks, and successfully applied in the study of the origin of varieties and the analysis of their pedigrees. <...> The research was carried out on 16 varieties from the collection of the All-Russian Scientific Research Institute of Viticulture and Winemaking (Novocherkassk) and the Russian ampelographic collection (Anapa). <...> Six SSR-markers basicly recommended for . fingerprinting were used. <...> DNA was extracted from young leaves of the apical shoots of 4-5 typical bushes. <...> Chardonnay and Cabernet Sauvignon were used as the reference cultivars. <...> Genetic distance matrix was constructed using the coefficients (indices) similarity of M. Nei and W. Li. <...> Based on the data of SSR-genotyping, estimation of the genetic similarity of studied varieties was performed using cluster analysis (UPGMA), and dendrograms were graphically constructed. <...> Data on the morphological characteristics of leaves and SSR-genotyping results were analyzed by means of principal coordinates (PCA). <...> DNA profiles of 16 local Don grapevine varieties were obtained using microsatellite loci VVMD5, VVMD7, VVMD27, VVS2, VrZAG62 and VrZAG79 with an automated genetic analyzer ABI Prism3130 («Applied Biosystems», USA). <...> In the studied Don varieties genotypes, six (for VVS2, VVMD5, VMD7, VrZAG62) and seven (for VVMD27, VrZAG79) alleles per locus were determined. <...> Cluster analysis allowed to divide the varieties into two main groups: one included Sibir’kovyi, Puhlyakovskii belyi, Sivolistnyi, Puhlyakovskii <...>