Slobodana Bajicha 1, 11185 Belgrade-Zemun, Serbia, e-mail radenovic@sbb.rs; 2Faculty of Physical Chemistry, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia, e-mail radenovic@sbb.rs; 3M.V. <...> Ogarev Mordovia State University, 68, Bol’shevistskaya ul., Saransk, Republic of Mordovia, 430005 Russia Received June 30, 2016 A b s t r a c t Modern biophysical methods have provided a breakthrough in investigations of the status and functions of the intact plants at the molecular level. <...> The infrared (IR) spectroscopy allows us to analyze the molecular composition and structure by recording the absorption of infrared radiation as a function of frequency of valent and deformation vibrations (wavenumber, cm-1) for chemical bonds. <...> We used the IR Fourier spectroscopy method (IR spectra with Fourier Transformation,) to investigated the grain composition in maize hybrids — ZP 341, ZP 434 and ZP 505 created at Maize Research Institute (Zemun Polje, Belgrade, Serbia). <...> The resulted spectra differed in peak number and intensity, and in oscillation frequency. <...> Particularly, there are 20 to 23 peaks and characteristic spectral bands within the wavenumber range of 400 to 4000 сm-1. <...> Characteristic spectral bands were analyzed for each hybrid with regard to absorption intensity in %, experimentally determined wavenumber in cm-1, and published wavenumber range. <...> A comparison of these peaks to reference IR spectra from databases revealed biogenic organic molecules: alcohols, amines, esters, alkanes, carboxylic acids, alkenes, aldehydes, ketones and esters in the studied grain hybrids. <...> In a typical IR spectrum of maize hybrid ZP 341 there were three most distinct bands with wavenumbers of 3400, 2900 and 1000 cm-1. <...> In general, Serbian hybrids are characterized by high quality, productivity and technological suitability. <...> The developed methodology for IR spectra recording and analysis in grain allows to reveal the composition and structure of biogenic compounds. <...> Keywords: maize hybrids, grain, molecular structural characteristics, infrared spectra, spectral bands Modern biophysical study methods have provided a significant breakthrough in the diagnostics of physical and functional condition of intact plants on the molecular level [1-9]. <...> Analysis of IR spectra allows to obtain important data on structural features of systems undergoing study <...>