Запорожский государственный медицинский университет, Запорожье, Украина The 2-mercaptobenzothiazole determination by UV spectrophotometric method in bulk Определение 2-меркаптобензотиазола УФ-спектрофотометрическим методом в субстанции ______________________ Abstract __________________________________________________________________________ A simple, rapid, sensitive and low cost UV-spectrophotometric method has been developed for quantification of 2-mercaptobenzothiazole in bulk and validated for linearity, range, accuracy, precision, ruggedness and robustness. <...> The valid maximum absorbance was recorded at the 324 nm in the concentration ranges 0.3–0.8 mg/100 mL of 2-MBT in water – methanol (1:1) solution after 20 min of stirring. <...> The limit of detection was found to be 0.09 mg/100 mL, while limit of quantification was 0.26 mg/100 mL. The calibration curve was linear with r2 =0.9986. <...> The proposed technique could be used for future studies of 2-MBT in pharmaceutical dosage forms. <...> Ключевые слова: 2-меркаптобензотиазол, валидация, УФ-спектрофотометрия. _________________________________________________________________________________________________ INTRODUCTION The antifungal resistance progressed each year against the widely used drugs, so it is always up-to-date to search for novel fungicidal agents or to investigate the old ones. 2-Mercaptobenzothiazole (2-MBT) is an important «Рецепт», 2016, том 19, № 3 339 The 2-mercaptobenzothiazole determination by UV spectrophotometric method in bulk commercial chemical, used in the production of rubber products and as a starting material to make other chemicals in USA and Europe (Fig. 1) [1]. <...> It is also a corrosion inhibitor in oils, greases, and cooling fluids; is added as a stabilizer in polyether polymers to resist damage by air and ozone [2]. <...> But, what is more important, it is effective antifungal agent [3, 4], also mentioned as inhibitor of dopamine β-hydroxylase [1]. <...> Namely, application of HPLC tandem mass spectrometry in positiveelectrospray ionization mode using isotope-dilution quantification [5], electrochemical [6] or UV [7] detection. <...> The capillary electrophoresis with amperometric detection for the simultaneous presence of 2-aminothiazole, 2-aminobenzothiazole and 2-MBT in river water [9]. <...> The simplest found one was UV spectrophotometry of chloroform extract from flotation liquors, obtained after acidifying it to less, than pH 2 or after adjusting the pH to 7–8 with ammonium acetate [13]. <...> Still, there was found no simple, fast and accurate method to determine quantity of 2-MBT in bulk in cheaper solvents <...>