Химия в интересах устойчивого развития 24 (2016) 555560 UDС 622.232;546.3 DOI: 10.15372/KhUR20160416 Synthesis and Safety Evaluation of Gold Nanoparticles in the in Vivo Experiment T. G. TOLSTIKOVA1, M. V. KHVOSTOV1, N. A. ZHUKOVA1, N. V. SHIKINA2 and Z. R. ISMAGILOV2,3 1Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia E-mail: tg_tolstikova@mail.ru 2Boreskov Institute of Catalysis, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia 3Institute of Coal Chemistry and Material Science, Federal Research Center of Coal and Coal Chemistry, Siberian Branch, Russian Academy of Sciences, Kemerovo, Russia E-mail: IsmagilovZR@iccms.sbras.ru Abstract In continuation of works on toxicology of nanocarbon materials, investigations of safety of colloidal gold nanoparticles in the 10 to 40 nm size range in two lines of mice (outbred and line C57BL/6j) were performed. <...> It was shown that in 24 h and 30 days after a single parenteral injection of colloidal gold nanoparticles in mice, the toxic action on indicators of peripheral blood, behaviour of animals, as well as internal organs was not revealed. <...> Key words: gold nanoparticles, toxicity, peripheral blood, behaviour, microscopic examination, outbred mouse, C57BL/6j mice INTRODUCTION Safety of nanomaterials and nanotechnologies is an important factor regulating the industrial production. <...> Given the peculiarities of the ingress of nanoparticles into the human body and animals, the main target organs are the tissues of the respiratory, interstitial, immune and skin systems. <...> In works by Ismagilov and Zarytova published in the last decade, the successful use of titan dioxide nanoparticles for the delivery therapeutic oligonucleotides into cells was shown. <...> The given task was posed on one hand, as the study of a more methodically deterministic object in the continuation of the works on nanocarbon materials, as well as in connection with a great concernment of the application of gold nano© Tolstikova T. G., Khvostov M. V., Zhukova N. A., Shikina N. V. and Ismagilov Z. R., 2016 555 556 T. G. TOLSTIKOVA et al. particles in various manufacture areas. <...> For gold nanoparticles, along with positive effects, negative effects are known, as the quantitative accumulation in the internal organs (liver, spleen, lungs <...>