Kostycheva, Ryazan, Russia 390044, e-mail alestarceva@yandex.ru Acknowledgements: Works on the isolation of rhizobacteria and the study of their antifungal properties, ability to produce auxins and various enzymes, as well as the effectiveness of endophytic and rhizobacteria in pot experiments were performed with the financial support of the Russian Science Foundation (project ¹ 14-16-00146), work on the efficacy of endophytic and rhizobacteria in field experiments with spring barley was made with state financial support of the leading universities of the Russian Federation, grant 074-U01. <...> Received April 24, 2015 A b s t r a c t We use an approach involving a combination of endophytic and rhizobacteria in the development of microbial preparations for crop production, which allows to create self sufficient plantmicrobe systems, enhancing agricultural production and reducing the environmental burden. <...> The article presents data on the properties of promising strains of endophytic and rhizobacteria and their efficiency in pot and field experiments. <...> Rhizobacteria were isolated from the roots of tomato plants (Solanum lycopersicum L.) cultivar Bella, endophytic bacteria from internal tissues of hogweed (Heracleum sphondylium L.) stem. <...> Identification of the studied bacterial strains was determined by nucleotide sequences of the 16S rRNA gene, and on cultural and biochemical properties. <...> Middle grade lettuce (Lactuca sativa L.) variety Eralash and early maturing variety Duro of radish (Raphanus sativus L.) were used in pot experiments. <...> It was shown that the Bacillus subtilis strain TR6 was active against all tested phytopathogenic fungi. <...> Zones of inhibition varied from 17.4 to 48.2 mm. Almost all of the studied strains were capable of producing auxins, except strain TR7. <...> Highest auxin production was observed in strains TR1 and TR9 — 12.3 and 20.1 µg/ml of medium respectively. <...> Three of the five studied strains (B. subtilis НС8, B. subtilis B2G and Azotobacter chroococcum AZ7) significantly (by 15.2-34.2 %) increased the biomass of the lettuce. <...> The strains of B. subtilis Ch-13 and B. subtilis TR6 increased the yield of lettuce but insignificantly. <...> Among the studied strains, B. subtilis НС8 should be noted, which significantly (by 15.2-42.1 %) increased harvest of radishes and lettuce in pot experiments. <...> Thus, the grain yield of barley variety Danuta (2-year field experiments <...>