Biology 2 (2016 9) 136-144 ~ ~ ~ УДК 579.6 Synthesis and Intracellular Degradation of P(3HB)/DEG Copolymers by Cupriavidus eutrophus B-10646 Natalia O. Zhila* Institute of Biophysics SB RAS 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia Siberian Federal University 79 Svobodny, Krasnoyarsk, 660041, Russia Received 28.12.2015, received in revised form 13.02.2016, accepted 01.05.2016 The study addresses synthesis and intracellular degradation of copolymers consisting of 3-hydroxybutyrate and diethylene glycol (P(3HB)/DEG) by Cupriavidus eutrophus В-10646. <...> DEG addition to the medium at concentrations of 10-30 g/L did not infl uence the yields of biomass and polymer. <...> During intracellular degradation, content of P(3HB)/DEG copolymers and homopolymer P(3HB) decreased at 1.1-1.2 and 1.8 times respectively. <...> Bacterial cells grown in the medium with DEG synthesized copolymer consisting of polymer fractions with low molecular weight (62-217 kDa) and high molecular weight (2810-4860 kDa), whose contents were 78.1-96.4 % and 3.6-21.9 %, respectively, depending on the culture growth phase. <...> DEG addition to the medium caused changes in the content of fatty acids of intracellular lipids, increasing their saturation. <...> All rights reserved * Corresponding author E-mail address: lhab@ibp.ru # 136 # Natalia O. Zhila. <...> Synthesis and Intracellular Degradation of P(3HB)/DEG Copolymers by Cupriavidus eutrophus B-10646 Синтез и внутриклеточная деградация сополимеров П(3ГБ)/ДЭГ в культуре природного штамма Cupriavidus eutrophus B-10646 Н.О. Жила Институт биофизики СО РАН Россия, 660036, Красноярск, Академгородок, 50/50 Сибирский федеральный университет Россия, 660041, Красноярск, пр. <...> Свободный, 79 Исследован синтез и внутриклеточная деградация диблок-сополимеров 3-гидроксибутирата и диэтиленгликоля (П(3ГБ)/ДЭГ) культурой Cupriavidus eutrophus В-10646. <...> Показано, что добавление ДЭГ в среду в концентрации 10-30 г/л не <...>