Biology 2 (2015 8) 220-235 ~ ~ ~ УДК 579.222 To the Question About Intracellular Polyhydroxybutyrate Degradation Natalia O. Zhilaa,b Galina S. Kalacheva*a,b , and Tatiana G. Volovaa,b 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia b Received 12.01.2015, received in revised form 19.02.2015, accepted 18.04.2015 The investigation of dynamics of 1,214 С-acetate fl ows was carried out in three different regimes of bacteria Cupriavidus eutrophus B-10646 biosynthesis on fructose and acetate: a) in the phase of accumulation of polyhydroxybutyrate; b) in the phase of intracellular PHB degradation and the synthesis of nitrogen compounds; c) in the phase of resynthesis of PHB. <...> It has been shown that in regime of PHB accumulation 80 % of labeled carbon was used for synthesis of PHB. <...> At the condition of PHB degradation both synthesis and degradation take place simultaneously. <...> This confi rms the cyclic nature of PHB methabolism. <...> All rights reserved * Corresponding author E-mail address: kalach@ibp.ru С-acetate, polyhydroxybutyrate, biosynthesis, aInstitute of Biophysics SB RAS Siberian Federal University 79 Svobodny, Krasnoyarsk, 660041, Russia # 220 # Natalia O. Zhila, Galina S. Kalacheva… <...> To the Question About Intracellular Polyhydroxybutyrate Degradation К вопросу о внутриклеточной деградации полигидроксибутирата Н.О. Жилаа,б , Г.С. Калачеваа,б , Т.Г. Воловаа,б аИнститут биофизики СО РАН Россия, 660036, Красноярск, Академгородок, 50/50 б Исследованы в динамике потоки меченого углеродного субстрата (1,214 Россия, 660041, Красноярск, пр. <...> Свободный, 79 С-ацетата) в меняющихся режимах биосинтеза бактерий Cupriavidus eutrophus B-10646 при росте на фруктозе и ацетате: а) в ходе накопления запасного соединения – полигидроксибутирата (ПГБ), б) эндогенной деградации ПГБ и синтеза азотсодержащих компонентов, в) ресинтеза ПГБ. <...> Показано, что при выращивании бактерий <...>