Biology 2 (2014 7) 161-173 ~ ~ ~ УДК 579.6 Characterization of Cupriavidus eutrophus В-10646 Culture Synthesizing Polyhydroxyalkanoates Grown on Sugars And Lipidic Substrates Natalia O. Zhilaa Tatiana G. Volovaa,b *, and Galina S. Kalachevaa aInstitute of Biophysics SB RAS 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia b Siberian Federal University 79 Svobodny, Krasnoyarsk, 660041, Russia Received 09.05.2014, received in revised form 24.05.2014, accepted 04.06.2014 Growth, polyhydroxyalkanoates (PHAs) accumulation and total fatty acid composition of the lipids of Cupriavidus eutrophus B-10646 were studied, using various carbon sources (fructose, glucose, oleic acid, sunfl ower seed oil). <...> The best substrates for biomass production (7.8-8.6 g/l) and polymer synthesis (7.3-7.9 g/l) were sugars and oleic acid. <...> Bacterial cells grown on sugars and sunfl ower seed oil synthesized only homopolymer poly(3-hydroxybutyrate). <...> 3-hydroxyvalerate (2.0-4.2 mol. %) was identifi ed in polymer when Cupriavidus eutrophus used oleic acid as sole carbon source. <...> Study of total fatty acid composition of lipids showed that major fatty acids were palmitic (16:0), palmitoleic (16:1ω7), and cis-vaccenic (18:1ω7) acids. <...> When carbohydrate substrate was replaced by oleic acid or sunfl ower seed oil, the proportion of oleic acid in the total fatty acids increased considerably. <...> In addition to that, the lipid fatty acids of bacterial cells grown on sunfl ower seed oil also contained linoleic acid, which is the major acid of sunfl ower seed oil. <...> Keywords: Cupriavidus eutrophus, fatty acids, polyhydroxyalkanoates, oleic acid, sunfl ower seed oil. <...> All rights reserved * Corresponding author E-mail address: lhab@ibp.ru # 161 # Natalia O. Zhila <...>