Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2012, № 6, p. 109-115 UDK 636/639:614.31:619 METHODS FOR SANITARY SURVEILLANCE OF LIVESTOCK PRODUCTION. <...> ENZYMOIMMUNOASSAY OF STREPTOMYCIN M.A. Burkin1, I.A. Gal’vidis1, G.P. Kononenko2 I.I. Mechnikov Research and Development Institute for Vaccines and Sera, RAMS, Moscow 105064, Russia e-mail: instmech@iitp.ru 2 All-Russia Research and Development Institute of Veterinary Sanitation, Hygiene and Ecologyl, RAAS, Moscow 123022, Russia e-mail: kononenkogp@mail.ru Received March 3, 2012 S umma r y The polyclonal rabbit antibodies against streptomycin conjugated with bovine serum albumin in reaction with diglycidyl ether of 1,4-butanediol presents high specificity to streptomycin and its similar structural analog — dihydrostreptomycin. <...> In the conditions of indirect competitive enzymoimmunoassay with immobilized antigens, heterologous to immunogene on protein carrier and methods of synthesis, the limit of streptomycin detection is 0.1 ng/ml. The authors consider the use of developed immunoenzyme test-systems for control of antibiotic contamination in milk and eggs with the limit of detection of 10 mkg/kg and in meat with the limit of 20 mkg/kg. Keywords: streptomycin, milk, eggs, meat, immunoassay. <...> Streptomycin (SM) is aminoglycoside antibiotic widely used for many years in our country and abroad for the treatment of acute infectious diseases of animals, despite its dangerous side effects such as allergic reactions, disorders of neuromuscular conduction, and ototoxicity (1 2). <...> In modern practice, the control of SM residues in animal products is often based on enzyme-linked immunosorbent assay (ELISA). <...> This method was implemented in several countries in the form of test systems of various types including biosensor technology (3-12). <...> Russian researchers also have proposed a test system based on direct ELISA and the evaluation test using immunochromatography for the control of SM in milk and dairy products (13, 14). <...> The purpose of this work was obtaining specific immunoreagents based on streptomycin, optimization of conditions of the indirect competitive solid-phase immunoassay, and assessing the applicability of this test system for the control of SM residues in milk, eggs and meat. <...> The experiment was performed using the following chemicals: streptomycin sulfate, diglycidyl ether of 1,4butanediol, adipic acid dihydrazide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI <...>