SEL’SKOKHOZYAISTVENNAYA BIOLOGIYA [AGRICULTURAL BIOLOGY], 2015, V. 50, ¹ 1, pp. 99-106 ISSN 2313-4836 (Online) Bacterial preparations for plant protection doi: 10.15389/agrobiology.2015.1.99rus doi: 10.15389/agrobiology.2015.1.99eng BACTERIAL STRAINS ANTAGONISTIC TO in vitro DEMONSTRATE DIFFERENT EFFICACY ON WHEAT SEEDLING IN GREEN HOUSE O.Yu. <...> KREMNEVA, A.M. ASATUROVA, M.D. ZHARNIKOVA, G.V. VOLKOVA Russian Academy of Agricultural Sciences, VNIIBZR, Krasnodar-39, 350039 Russia, e-mail kremenoks@mail.ru Supported by Grant of the President of the Russian Federation and the EurAsEU International Program «Innovation biotechnologies» of the Ministry of Education and Science of the Russian Federation A b s t r a c t Yellow leaf spot is a wide spread diseases of soft and hard wheat. <...> Numerous publications and the authors' own research show that its epiphytotics occur in different countries (Australia, Canada, USA, India, England, Belgium, Romania, Czech Republic, Kazakhstan) with crop losses reaching 65 %. <...> Development and application of new high-performance environmentally friendly biological products is regarded as one of the most effective biologized approach to wheat protection against the disease. <...> In searching potential bacterial agents for use as protective means, their in vitro antagonistic activity should be accompanied by the ability to provide effective protection for seeds and seedlings. <...> Herein we studied the repression of yellow spot development during early stages of plant vegetation in a greenhouse as influenced by the bacterial strains which were in vitro antagonistic to Drechsler. <...> Winter wheat cultivar Bat’ko susceptible to the pathogen was used as test plant. <...> BZR 417 from the collection of All-Russian Research Institute of Biological Plant Protection were used as candidate bio agents. <...> For comparison, liquid Fitosporin-M was used as a biological preparation (LLC Scientific Innovation Enterprise «BashInkom», Russia) and Prozaro emulsion concentrate was used as a chemical fungicide («Bayer CropScience», Germany). <...> Liquid cultures of antagonistic bacterial strains were applied in three modes, namely before inoculation (prophylactic treatment), at early signs of the disease (on the day 3 after inoculation) and by their combination. <...> All treatments were performed with inlaying and without inlaying grain with liquid bacterial culture. <...> BZR 417 showed considerable biological efficacy of leaf BZR <...>