AGRICULTURAL BIOLOGY, 2015, V. 50, ¹ 3, pp. 361-368 ISSN 2412-0324 (English ed. Online) (SEL’SKOKHOZYAISTVENNAYA BIOLOGIYA) ISSN 0131-6397 (Russian ed. Print) ISSN 2313-4836 (Russian ed. Online) Ecological approach to developing microbial biologicals doi: 10.15389/agrobiology.2015.3.361rus doi: 10.15389/agrobiology.2015.3.361eng EFFICIENCY OF BATSIKOL BASED ON A NEW STRAIN var. OF PHYTOPHAGOUS PESTS AND PHYTOPATHOGENS S.D. GRISHECHKINA, V.P. ERMOLOVA Federal Agency of Scientific Organizations, 3, sh. <...> Microorganisms of different origin are used for the production of biological preparations pathogenic to pests. <...> The preparations based on entomopathogenic bacteria (Bt) are of the most interest due to their selective action, safety for humans, warm-blooded animals, beneficial insects and the environment. <...> The departmental collection of beneficial microorganisms for agricultural purposes (RCAM collection, St. Petersburg) stores with known cultural, morphological, physiological, biochemical, technological, insecticidal and other properties. <...> One of the priorities of collection strains is var. preparation Batsikol and has a specific effect on (BtH10) ¹ 25, which bases the and antifungal activity against a number of pathogenic fungi. <...> To obtain qualitative preparation with a maximum accumulation of exotoxin and high titer we need to create optimal conditions for cultivation and improve the formulation of nutrient media for microorganisms-producers of biological preparations. <...> The objective of our research was to choose nutrient medium, work out cultivation regimes (aeration, temperature, pH, dosage, form, and age of inoculum) of strain BtH10 ¹ 25, and to determine its technological effectiveness, insecticidal and antifungal activity as well as the extent of exotoxin formation in test objects. <...> The best results were obtained in the pea flour medium, instead of soy flour: spores titer in liquid culture was 4½109/ml, activity to housefly (exotoxin content) was LC50 3.1 mkl/g feed, the inhibitory activity was 70.0 % to and 75.0 % to . <...> It was found that barley, oat flour, and mixtures of soybean and barley flour could be used for the preparation production. <...> The optimal development of the culture was observed at a ratio of medium volume-to-air from 1:30 to 1:15, and pH of the nutrient medium 6.65-7.20. <...> Liquid culture media and the spore culture rinse can be used as an inoculum. <...> Laboratory and field evaluation <...>