Dubrovitsy, Podolsk Region, Moscow Province, 142132 Russia, e-mail vijmikrob@mail.ru A b s t r a c t According to WHO reports, since 2000 a microorganism resistance to antimicrobials has become a serious threat to global public health. <...> Thereby, more strict control for used antibiotics and novel antibacterial substances are considered helpful. <...> Of that, the development of new antimicrobial compounds seems to be most perspective, seeing the high yielding animals are much more susceptible to diseases, and animal products are poorly stored because of microbial contamination. <...> Among compounds possessing antimicrobial properties the dihydroquercetin, a bioflavonoid is of special interest due to wide range of biological activity, including antioxidant activity. <...> Dihydroquercetin is used widely in food industry and medicine, but in animal farming its use is a novel project aimed to provide for animal welfare and quality of livestock products. <...> We compared in vitro antimicrobial effect of different antibiotics (tetracycline, chloramphenicol, streptomycin, bacitracin, grisin, benzyl penicillin at 3.0, 5.0, 10.0, 16.0, 19.0, 24.0 and 48.0 g/ml each) and 0.5, 1.0, 2,0 and 5.0 % dihydroquercetin to pathogenic, opportunistic, and probiotic microorganism АТСС 14990, ( VL-613, ) АТСС 4698, diameters of growth inhibition zone (D) were measured. . <...> World Health Organization (WHO) notes that microbial resistance poses a growing threat in the last twenty years. <...> This resistance develops due to injudicious antibiotic use in healthcare and animal husbandry [1, 2]. <...> WHO report on April 30, 2014, presented data on microbial resistance to antimicrobial drugs in more than 114 European and African countries for the first time (http://www.who.int/drug-resistance/documents/surveillancereport/en/). <...> However, the use of antimicrobial drugs in large animal populations may result in spread of bacteria resistant to antibacterial drugs and may cause drug-resistant infections [3-6]. <...> The higher number of resistant bacterial strains, which are common infectious pathogens for humans and animals, has the greatest epidemiological importance. <...> Humans may be infected through contaminated food, after direct contact with animals, or though environment. <...> In EU countries, resistance to erythromycin is unevenly distributed among strains isolated from poultry and pigs <...>